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By Gérard Ailhaud

ISBN-10: 0896037479

ISBN-13: 9780896037472

ISBN-10: 1592592317

ISBN-13: 9781592592319

Adipose tissue is now well-known as a widely-dispersed secretory organ that performs an important function in weight problems. Ailhaud (U. de Nice-Sophia Antipolis, great, France) and a workforce of eu and US laboratory specialists and clinicians aspect the category, key thoughts hired, and their capability difficulties, within the in vivo and in vitro research of brown and white fats tissues and cells from the dietary, physiological, and molecular degrees. significant remedy is accorded to the isolation, subcellular fractionation, and gene move into mature adipocytes, in addition to metabolic elements. Illustrations comprise microscopy learn effects, and a guy donning a transportable detector for measuring adipose tissue blood movement.

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1985) Human brown adipose cells in culture. Exp. Cell Res. 159, 261–266. 39. , and Zancanaro, C. (1986) Ultrastructure of brown adipocytes mitochondria in cell culture from explants. J. Submicrosc. Cytol. 18, 625–627. 40. , and Bosello, O. (1983) Ultrastructural features of cultured mature adipocytes precursors from adipose tissue in multiple symmetric lipomatosis. Ultrastruct. Pathol. 5, 145–152. 41. , and Björntorp, P. (1987) Effects of noradrenaline exposure on rat brown adipocytes in cultures.

Biometr. Zschr. 12, 156. 19. Williams, M. A. (1977) Quantitative methods in biology, in Practical Methods in Electron Microscopy, vol. 6, (Glauert, A. ), North-Holland, Amsterdam, The Netherlands. 20. Hayat, M. A. (1986) Basic Techniques for Transmission Electron Microscopy, Academic Press, New York. 21. Thompson, S. W. (1966) Selected Histochemical and Histopathological Methods, Thomas Publisher, Springfield, IL. 22. Hsu, S. , and Fanger, H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: a comparison between ABC and unlabeled antibody (PAP) procedures.

Embedding Tissue specimens are embedded in paraffin previously melted and filtered (paraffin is solid at ambient temperature and melts over 50°C). The embedding procedure requires sequential washes with saline solution to remove the fixative and tissue dehydration by multiple passages in ascending alcohol concentrations. 85% saline/100% EtOH 1:1 v/v, 70% EtOH, 85% EtOH, 95% EtOH, and 100% EtOH, with occasional shaking. Samples are then immersed in xylol–paraffin, 1:1, at 58°C and, in paraffin at 58°C: this step is performed 3×.

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Adipose Tissue Protocols by Gérard Ailhaud

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