By Gérard Ailhaud
Adipose tissue is now well-known as a widely-dispersed secretory organ that performs an important function in weight problems. Ailhaud (U. de Nice-Sophia Antipolis, great, France) and a workforce of eu and US laboratory specialists and clinicians aspect the category, key thoughts hired, and their capability difficulties, within the in vivo and in vitro research of brown and white fats tissues and cells from the dietary, physiological, and molecular degrees. significant remedy is accorded to the isolation, subcellular fractionation, and gene move into mature adipocytes, in addition to metabolic elements. Illustrations comprise microscopy learn effects, and a guy donning a transportable detector for measuring adipose tissue blood movement.
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Extra info for Adipose Tissue Protocols
1985) Human brown adipose cells in culture. Exp. Cell Res. 159, 261–266. 39. , and Zancanaro, C. (1986) Ultrastructure of brown adipocytes mitochondria in cell culture from explants. J. Submicrosc. Cytol. 18, 625–627. 40. , and Bosello, O. (1983) Ultrastructural features of cultured mature adipocytes precursors from adipose tissue in multiple symmetric lipomatosis. Ultrastruct. Pathol. 5, 145–152. 41. , and Björntorp, P. (1987) Effects of noradrenaline exposure on rat brown adipocytes in cultures.
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Embedding Tissue specimens are embedded in paraffin previously melted and filtered (paraffin is solid at ambient temperature and melts over 50°C). The embedding procedure requires sequential washes with saline solution to remove the fixative and tissue dehydration by multiple passages in ascending alcohol concentrations. 85% saline/100% EtOH 1:1 v/v, 70% EtOH, 85% EtOH, 95% EtOH, and 100% EtOH, with occasional shaking. Samples are then immersed in xylol–paraffin, 1:1, at 58°C and, in paraffin at 58°C: this step is performed 3×.
Adipose Tissue Protocols by Gérard Ailhaud