Get Adipose Tissue Protocols PDF

By Gérard Ailhaud

ISBN-10: 0896037479

ISBN-13: 9780896037472

ISBN-10: 1592592317

ISBN-13: 9781592592319

Adipose tissue is now well-known as a widely-dispersed secretory organ that performs an important function in weight problems. Ailhaud (U. de Nice-Sophia Antipolis, great, France) and a workforce of eu and US laboratory specialists and clinicians aspect the category, key thoughts hired, and their capability difficulties, within the in vivo and in vitro research of brown and white fats tissues and cells from the dietary, physiological, and molecular degrees. significant remedy is accorded to the isolation, subcellular fractionation, and gene move into mature adipocytes, in addition to metabolic elements. Illustrations comprise microscopy learn effects, and a guy donning a transportable detector for measuring adipose tissue blood movement.

Show description

Read or Download Adipose Tissue Protocols PDF

Best endocrinology books

The Mathematics of Sex: How Biology and Society Conspire to by Stephen J. Ceci, Wendy M. Williams PDF

Approximately 1/2 all physicians and biologists are adult females, as are the vast majority of new psychologists, veterinarians, and dentists, suggesting that girls have accomplished equality with males within the staff. however the ranks of execs in math-intensive careers stay lopsidedly male; as much as ninety three% of tenure-track educational positions in essentially the most mathematically-oriented fields are held by way of males.

Download PDF by Joseph Lorenzo, Mark Horowitz, Yongwon Choi, Hiroshi: Osteoimmunology: Interactions of the Immune and Skeletal

Bone and the immune procedure are either advanced tissues, which, respectively, keep an eye on the skeleton and the body's responses to invading pathogens. serious interactions among those organ platforms usually occur, particularly in the improvement of immune cells within the bone marrow and for the functionality of bone cells in healthiness and sickness.

Principles of Bone Biology, Two-Volume Set, Third Edition by John P. Bilezikian, Lawrence G. Raisz, T. John Martin PDF

Offers the main entire, authoritative reference at the research of bone biology and similar ailments. it's the crucial source for a person inquisitive about the research of bone biology. it's the so much complete, entire, up to date resource of data on all points of bones and bone biology in a single handy resource.

New PDF release: Natriuretic Peptides

Glossy cardiovascular technology has produced a progressive new thought: the guts acts now not in basic terms as a pump, yet as a "gland", that's as a regulator of circulatory homeostasis and salt-water stability. This booklet combines the classical heamodynamic view with the hot neuro-hormonal paradigm, in all its power medical implications.

Extra info for Adipose Tissue Protocols

Example text

1985) Human brown adipose cells in culture. Exp. Cell Res. 159, 261–266. 39. , and Zancanaro, C. (1986) Ultrastructure of brown adipocytes mitochondria in cell culture from explants. J. Submicrosc. Cytol. 18, 625–627. 40. , and Bosello, O. (1983) Ultrastructural features of cultured mature adipocytes precursors from adipose tissue in multiple symmetric lipomatosis. Ultrastruct. Pathol. 5, 145–152. 41. , and Björntorp, P. (1987) Effects of noradrenaline exposure on rat brown adipocytes in cultures.

Biometr. Zschr. 12, 156. 19. Williams, M. A. (1977) Quantitative methods in biology, in Practical Methods in Electron Microscopy, vol. 6, (Glauert, A. ), North-Holland, Amsterdam, The Netherlands. 20. Hayat, M. A. (1986) Basic Techniques for Transmission Electron Microscopy, Academic Press, New York. 21. Thompson, S. W. (1966) Selected Histochemical and Histopathological Methods, Thomas Publisher, Springfield, IL. 22. Hsu, S. , and Fanger, H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: a comparison between ABC and unlabeled antibody (PAP) procedures.

Embedding Tissue specimens are embedded in paraffin previously melted and filtered (paraffin is solid at ambient temperature and melts over 50°C). The embedding procedure requires sequential washes with saline solution to remove the fixative and tissue dehydration by multiple passages in ascending alcohol concentrations. 85% saline/100% EtOH 1:1 v/v, 70% EtOH, 85% EtOH, 95% EtOH, and 100% EtOH, with occasional shaking. Samples are then immersed in xylol–paraffin, 1:1, at 58°C and, in paraffin at 58°C: this step is performed 3×.

Download PDF sample

Adipose Tissue Protocols by Gérard Ailhaud


by Mark
4.1

Rated 4.30 of 5 – based on 43 votes